α-Ketoglutarate Dehydrogenase Activity Assay Kit (Colorimetric) (ab185440)
Key features and details
- Assay type: Semi-quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 100 µU
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests KGDH Assay Buffer 1 x 25ml KGDH Developer 1 vial KGDH Positive Control 1 x 50µl KGDH Substrate 1 vial NADH Standard ( lyophilized) Yellow 1 vial -
Research areas
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Function
The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). -
Sequence similarities
Belongs to the alpha-ketoglutarate dehydrogenase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Alternative names
- 2 oxoglutarate dehydrogenase
- 2 oxoglutarate dehydrogenase complex component E1
- 2 oxoglutarate dehydrogenase mitochondrial
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Images
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NADH Standard Curve
This is example data only.
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alpha-Ketoglutarate Dehydrogenase activity in various samplesα-Ketoglutarate Dehydrogenase activity in rat heart (75 µg) and liver lysates (100 µg). Assays were performed following the kit protocol. This is example data only.
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alpha-Ketoglutarate Dehydrogenase specific activity in various samplesα-Ketoglutarate Dehydrogenase specific activity was calculated in rat heart lysate (75 µg), yeast mitochondria prepared from S. Cerevisiae(10 µg) and in rat liver lysate (100 µg). Assays were performed following the kit protocol. This is example data only.