Anti-hnRNP A2B1 antibody (ab223237)
Key features and details
- Goat polyclonal to hnRNP A2B1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-hnRNP A2B1 antibody
See all hnRNP A2B1 primary antibodies -
Description
Goat polyclonal to hnRNP A2B1 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Pig, Orangutan
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Immunogen
Synthetic peptide corresponding to Human hnRNP A2B1 aa 100-200 (internal sequence) (Cysteine residue). NP_002128.1; NP_112533.1.
Database link: P22626 -
Positive control
- WB: MCF7 cell lysate; NIH/3T3 nuclear lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab223237 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody (ab223237)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human small intestine staining hnRNP with ab223237 at 5 μg/ml
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Western blot - Anti-hnRNP A2B1 antibody (ab223237)Lane 1 : Anti-hnRNP A2B1 antibody (ab223237) at 0.1 µg/ml
Lanes 2-3 : Anti-hnRNP A2B1 antibody (ab223237) at 0.01 µg/ml
Lane 1 : NIH3T3 cell lysate (in RIPA buffer)
Lane 2 : Nuclear NIH3T3 cell lysate (in RIPA buffer)
Lane 3 : NIH3T3 cell lysate (in RIPA buffer) with Peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 36 kDaDetected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody (ab223237)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human testies staining hnRNP with ab223237 at 5 μg/ml
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Western blot - Anti-hnRNP A2B1 antibody (ab223237)Anti-hnRNP A2B1 antibody (ab223237) at 0.3 µg/ml + MCF7 (human breast adenocarcinoma cell line) cell lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 36 kDa
Observed band size: 36 kDaPrimary incubation was 1 hour.
