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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing

Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] - BSA and Azide free (ab248575)

Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] - BSA and Azide free (ab248575)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPNCIR152] to hnRNP C1 + C2/HNRNPC - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] - BSA and Azide free
    See all hnRNP C1 + C2/HNRNPC primary antibodies
  • Description

    Rabbit monoclonal [EPNCIR152] to hnRNP C1 + C2/HNRNPC - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody is specific to isoform C2. 

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248575 is the carrier-free version of ab133607.

    This antibody was developed as part of a collaboration between the National Cancer Institute's Center for Cancer Research and the lab of Mirit Aladjem. View antibodies from NCI Center for Cancer Research Collaboration.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPNCIR152
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Images

  • Western blot - Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] - BSA and Azide free (ab248575)
    Western blot - Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] - BSA and Azide free (ab248575)
    All lanes : Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] (ab133607) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 33 kDa



    This data was developed using ab133607, the same antibody clone in a different buffer formulation.

    Actual band size: 42 kDa.

  • Immunocytochemistry - Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] - BSA and Azide free (ab248575)
    Immunocytochemistry - Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] - BSA and Azide free (ab248575)
    This data was developed using ab133607, the same antibody clone in a different buffer formulation.Immunofluorescence staining of MCF-7 cells with purified ab133607 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] - BSA and Azide free (ab248575)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] - BSA and Azide free (ab248575)
    This data was developed using ab133607, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling hnRNP C1 + C2/HNRNPC with ab133607 antibody at a dilution of 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] - BSA and Azide free (ab248575)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] - BSA and Azide free (ab248575)
    This data was developed using ab133607, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labelling hnRNP C1 + C2/HNRNPC with ab133607 antibody at a dilution of 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] - BSA and Azide free (ab248575)
    Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] - BSA and Azide free (ab248575)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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