Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] - BSA and Azide free (ab248575)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR152] to hnRNP C1 + C2/HNRNPC - BSA and Azide free
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] - BSA and Azide free
See all hnRNP C1 + C2/HNRNPC primary antibodies -
Description
Rabbit monoclonal [EPNCIR152] to hnRNP C1 + C2/HNRNPC - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody is specific to isoform C2.
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Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248575 is the carrier-free version of ab133607.
This antibody was developed as part of a collaboration between the National Cancer Institute's Center for Cancer Research and the lab of Mirit Aladjem. View antibodies from NCI Center for Cancer Research Collaboration.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPNCIR152 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] (ab133607) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : K562 cell lysate
Lane 5 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 33 kDaThis data was developed using ab133607, the same antibody clone in a different buffer formulation.
Actual band size: 42 kDa.
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This data was developed using ab133607, the same antibody clone in a different buffer formulation.Immunofluorescence staining of MCF-7 cells with purified ab133607 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.
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This data was developed using ab133607, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling hnRNP C1 + C2/HNRNPC with ab133607 antibody at a dilution of 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab133607, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labelling hnRNP C1 + C2/HNRNPC with ab133607 antibody at a dilution of 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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