Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free (ab250883)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13374-2] to Elav-type RNA-binding protein ETR3 - BSA and Azide free
  • Suitable for: IHC-P, ICC, IP, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free
    See all Elav-type RNA-binding protein ETR3 primary antibodies

  • Description

    Rabbit monoclonal [EPR13374-2] to Elav-type RNA-binding protein ETR3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250883 is the carrier-free version of ab186430. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250883 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free (ab250883)
    Western blot - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free (ab250883)
    All lanes : Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430) at 1/5000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa



    This data was developed using ab186430, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free (ab250883)
    Immunocytochemistry - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free (ab250883)

    This data was developed using ab186430, the same antibody clone in a different buffer formulation.

    ab186430 staining Elav-type RNA-binding protein ETR3 in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilised with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain. Negative control 1:PBS only.
  • Western blot - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free (ab250883)
    Western blot - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free (ab250883)
    All lanes : Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430) at 1/5000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa



    This data was developed using ab186430, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free (ab250883)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free (ab250883)

    This data was developed using ab186430, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free (ab250883)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free (ab250883)
    This data was developed using ab186430, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free (ab250883)
    Immunocytochemistry - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free (ab250883)
    This data was developed using ab186430, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/500 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
  • Immunocytochemistry - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free (ab250883)
    Immunocytochemistry - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free (ab250883)
    This data was developed using ab186430, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/500 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
  • Flow Cytometry - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free (ab250883)
    Flow Cytometry - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free (ab250883)
    This data was developed using ab186430, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/270 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
  • Immunoprecipitation - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free (ab250883)
    Immunoprecipitation - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free (ab250883)
    This data was developed using ab186430, the same antibody clone in a different buffer formulation.Western blot analysis of Elav-type RNA-binding protein ETR3 in HeLa cell lysate immunoprecipitated with ab186430 at 1/50 dilution (Lane 1). Lane 2: PBS instead of HeLa lysate.Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
  • Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free (ab250883)
    Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free (ab250883)

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