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Epigenetics and Nuclear Signaling DNA / RNA Translation tRNAs

Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961)

Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [YAR5H08] to Tyrosyl tRNA synthetase/TyrRS
  • Suitable for: WB, IP, ICC
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08]
    See all Tyrosyl tRNA synthetase/TyrRS primary antibodies
  • Description

    Mouse monoclonal [YAR5H08] to Tyrosyl tRNA synthetase/TyrRS
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IP, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human Tyrosyl tRNA synthetase/TyrRS.

  • Positive control

    • HeLa cells and cell lysate and NIH3T3 cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 1% BSA, 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Dibasic monohydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab50961 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • Clonality

    Monoclonal
  • Clone number

    YAR5H08
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNAs
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNA synthetase

Images

  • Western blot - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961)
    Western blot - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961)
    Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961) at 1/100 dilution + HeLa whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 58 kDa
    Observed band size: 58 kDa

  • Immunoprecipitation - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961)
    Immunoprecipitation - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961)
    Immunoprecipititation analysis using 10ug ab50961 per 500ug RIPA HeLa cell lysate. Immunoblot using ab50961 at 1/100 dilution and TrueBlotTM anti-mouse IgG at 1/1000 as secondary antibody.
  • Western blot - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961)
    Western blot - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961)
    Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961) at 1/100 dilution + NIH3T3 whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 58 kDa
    Observed band size: 58 kDa

  • Immunocytochemistry - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961)
    Immunocytochemistry - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961)
    ab50961 at 1/10 dilution staining human YARS in HeLa cells by Immunocytochemistry. AlexaFluor 488 Goat anti-mouse IgG at 1/200 as secondary.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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