Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (ab237969)

Key features and details

  • Mouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Human, Pig
  • Isotype: IgG1

Overview

  • Product name

    Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free
    See all alpha 1 Sodium Potassium ATPase primary antibodies

  • Description

    Mouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human, Pig
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Dog, Xenopus laevis, Monkey

  • Immunogen

    Full length native protein (purified) corresponding to Rabbit alpha 1 Sodium Potassium ATPase. Rabbit renal outermedulla.

  • Positive control

    • This antibody gave a positive signal in HEK293 whole cell lysate and in the following tissue lysates: human kidney, rabbit heart, human brain and human brain membrane.This antibody also gave a positive signal in Porcine proximal tubule lysate.

  • General notes

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine, and sodium azide (ab237969).

    This antibody clone [464.6] is manufactured by Abcam.

    If you require this antibody in a different buffer formulation or a different conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    IgG fraction

  • Clonality

    Monoclonal

  • Clone number

    464.6

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Images

  • Immunocytochemistry - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (ab237969)
    Immunocytochemistry - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (ab237969)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab7671)

    ab7671 staining alpha 1 Sodium Potassium ATPase in Hek293 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab7671 at 1?g/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 100% methanol (5 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a single confocal section is shown.

  • Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (ab237969)
    Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (ab237969)
    Lanes 1-3 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 1 µg/ml
    Lanes 4-6 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 2 µg/ml
    Lanes 7-9 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 5 µg/ml

    Lanes 1 & 4 & 7 : ab29466
    Lanes 2 & 5 & 8 : ab27253
    Lanes 3 & 6 & 9 : ab7942

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 113 kDa


    Exposure time: 3 minutes


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (ab7671).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (ab237969)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (ab237969)

    IHC image of Ab7671 staining in Human Kidney Carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab7671, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (ab7671).

  • Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (ab237969)
    Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (ab237969)
    All lanes : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 10 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : ab30203
    Lane 3 : ab29072
    Lane 4 : ab29466
    Lane 5 : Brain (Human) Membrane Lysate - adult normal tissue

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP), at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 113 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (ab7671).

  • Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (ab237969)
    Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (ab237969)
    Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 1/5000 dilution + Porcine proximal tubule lysate

    Predicted band size: 113 kDa



    Western blot analysis detecting Na, K-ATP-ase (alpha) in porcine proximal tubule protein, using Ab7671. Band is at 112 kDa.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (ab7671).

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