Anti-Coxiella burnetii Phase 2 IgG ELISA Kit (Q-Fever) (ab178639)
Key features and details
- Sensitivity: 90 %
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Overview
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Product name
Anti-Coxiella burnetii Phase 2 IgG ELISA Kit (Q-Fever)
See all Coxiella burnetii IgG kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Greyzone 15 3.4% Pos serum 16 2.6% Inter-assay Sample n Mean SD CV% Greyzone 5 10.1NTU/ml 10.8% Pos serum 16 19.5NTU/ml 1.7% -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Indirect -
Sensitivity
> 90 % -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s anti-Coxiella burnetii (Q-Fever) Phase 2 IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Coxiella burnetii in Human serum.
A 96-well plate has been precoated with Coxiella burnetii antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Coxiella burnetii antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Coxiella burnetii IgG sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover Foil 1 unit Coxiella burnetii Anti-IgG HRP Conjugate 1 x 20ml Coxiella burnetii IgG Cut-off Control 1 x 3ml Coxiella burnetii IgG Negative Control 1 x 2ml Coxiella burnetii IgG Positive Control 1 x 2ml Coxiella burnetii Phase 2 (IgG) Coated Microplate (12 x 8 wells) 1 unit IgG Sample Diluent 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
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Relevance
Coxiella burnetii is an obligate intracellular bacterial pathogen, and is the causative agent of Q fever. The genus Coxiella is morphologically similar to Rickettsia, but with a variety of genetic and physiological differences. C. burnetii is a small Gram-negative bacterium that is highly resistant to environmental stresses such as high temperature, osmotic pressure, and ultraviolet light. These characteristics are attributed to a small cell variant (SCV) form of the organism that is part of a biphasic developmental cycle, including a more metabolically and replicatively active large cell variant (LCV) form. It can survive standard disinfectants, and is resistant to many other environmental changes like those presented in the phagolysosome.
Images
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Serologic ELISA assay principleSpecific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
