Anti-hnRNP C1 + C2/HNRNPC antibody [4F4] (ab10294)
![Anti-hnRNP C1 + C2/HNRNPC antibody [4F4] (ab10294)](https://www.abcam.com/ps/products/10/ab10294/Images/ab10294-17876-anti-hnrnp-c1-c2-antibody-4f4-immunohistochemistry.jpg "Anti-hnRNP C1 + C2/HNRNPC antibody [4F4] (ab10294)")
Key features and details
- Mouse monoclonal [4F4] to hnRNP C1 + C2/HNRNPC
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-hnRNP C1 + C2/HNRNPC antibody [4F4]
See all hnRNP C1 + C2/HNRNPC primary antibodies -
Description
Mouse monoclonal [4F4] to hnRNP C1 + C2/HNRNPC -
Host species
Mouse -
Specificity
This antibody recognizes the two hnRNP C proteins of 41kDa (C1) and 43kDa (C2) in human cells, and corresponding proteins in monkey, hamster and chicken.
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Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human hnRNP C1 + C2/HNRNPC. RNPs eluted from oligo (dT) cellulose column.
Database link: P07910 -
Positive control
- IHC-P: Human placenta tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Protein A purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
4F4 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1 + C2/HNRNPC antibody [4F4] (ab10294)ab10294 (1µg/ml) staining hnRNP in human placenta, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
