Anti-KIAA0652/ATG13 antibody (ab240912)
Key features and details
- Rabbit polyclonal to KIAA0652/ATG13
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-KIAA0652/ATG13 antibody
See all KIAA0652/ATG13 primary antibodies -
Description
Rabbit polyclonal to KIAA0652/ATG13 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseWB Human
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Immunogen
Synthetic peptide corresponding to Human KIAA0652/ATG13 (internal sequence). From the mid-protein range of NP_001136145.1
Database link: O75143 -
Positive control
- WB: HEK-293 cell lysate. ICC/IF: C2C12 cells.
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General notes
This product was previously labelled as KIAA0652
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KIAA0652/ATG13 antibody (ab240912)Anti-KIAA0652/ATG13 antibody (ab240912) at 1/1000 dilution (1 hour at RT) + HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit-HRP for 1 hour at RT at 1/2000 dilution
Predicted band size: 57 kDaBlock: 5% milk + TBST for 1 hour at RT.
Color Development: TMB solution for 12 minutes at RT.
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Immunocytochemistry/ Immunofluorescence - Anti-KIAA0652/ATG13 antibody (ab240912)Immunocytochemistry/Immunofluorescence analysis of C2C12 (mouse myoblast cell line) cells using ab240912 at a 1:100 dilution for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT. Secondary antibody used is a Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at RT.
(Left panel) DAPI (blue) nuclear stain (Middle panel) ab240912 (Right panel) Composite.
