Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KIAA0652/ATG13 antibody [EPR19601] - BSA and Azide free (ab251347)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19601] to KIAA0652/ATG13 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-KIAA0652/ATG13 antibody [EPR19601] - BSA and Azide free
    See all KIAA0652/ATG13 primary antibodies

  • Description

    Rabbit monoclonal [EPR19601] to KIAA0652/ATG13 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251347 is the carrier-free version of ab201467.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] - BSA and Azide free (ab251347)
    Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] - BSA and Azide free (ab251347)
    All lanes : Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 3 : A20 (Mouse reticulum sarcoma cell line) whole cell lysate at 20 µg
    Lane 4 : A-673 (Human muscle Ewing's Sarcoma cell line) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 56 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab201467, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-3: 30 seconds; Lane 4: 3 minutes.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 18936157).

  • Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] - BSA and Azide free (ab251347)
    Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] - BSA and Azide free (ab251347)
    All lanes : Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467) at 1/1000 dilution

    Lane 1 : Human cerebellum lysate at 20 µg
    Lane 2 : Human hippocampus lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 56 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab201467, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] - BSA and Azide free (ab251347)
    Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] - BSA and Azide free (ab251347)
    All lanes : Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 5 : Mouse brain tissue lysate
    Lane 6 : Rat brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 56 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab201467, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] - BSA and Azide free (ab251347)
    Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] - BSA and Azide free (ab251347)
    All lanes : Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cytoplasmic fraction lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear fraction lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 56 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This data was developed using ab201467, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-KIAA0652/ATG13 antibody [EPR19601] - BSA and Azide free (ab251347)
    Flow Cytometry - Anti-KIAA0652/ATG13 antibody [EPR19601] - BSA and Azide free (ab251347)
    This data was developed using ab201467, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling KIAA0652/ATG13 with ab201467 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
  • Immunoprecipitation - Anti-KIAA0652/ATG13 antibody [EPR19601] - BSA and Azide free (ab251347)
    Immunoprecipitation - Anti-KIAA0652/ATG13 antibody [EPR19601] - BSA and Azide free (ab251347)
    This data was developed using ab201467, the same antibody clone in a different buffer formulation.KIAA0652/ATG13 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab201467 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab201467 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: HeLa whole cell lysate 10µg (Input). Lane 2: ab201467 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab201467 in HeLa whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds.
  • Anti-KIAA0652/ATG13 antibody [EPR19601] - BSA and Azide free (ab251347)
    Anti-KIAA0652/ATG13 antibody [EPR19601] - BSA and Azide free (ab251347)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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