Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19601] to KIAA0652/ATG13
Suitable for: Flow Cyt, WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-KIAA0652/ATG13 antibody [EPR19601]
See all KIAA0652/ATG13 primary antibodies
Description

Rabbit monoclonal [EPR19601] to KIAA0652/ATG13
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, MCF7, A29, A-673, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; human cerebellum and hippocampus lysates; mouse and rat brain lysates; HeLa cytoplasmic fraction lysate. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate.
General notes
This product was previously labelled as KIAA0652

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19601
Isotype

IgG
Research areas

Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467)

All lanes : Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467) at 1/1000 dilution

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 3 : A20 (Mouse reticulum sarcoma cell line) whole cell lysate at 20 µg
Lane 4 : A-673 (Human muscle Ewing's Sarcoma cell line) whole cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 56 kDa
Observed band size: 70 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1-3: 30 seconds; Lane 4: 3 minutes.

The molecular weight observed is consistent with what has been described in the literature (PMID:18936157).
Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467)

All lanes : Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467) at 1/1000 dilution

Lane 1 : Human cerebellum lysate at 20 µg
Lane 2 : Human hippocampus lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 56 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467)

All lanes : Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467) at 1/1000 dilution

Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 5 : Mouse brain lysate
Lane 6 : Rat brain lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 56 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467)

All lanes : Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467) at 1/1000 dilution

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cytoplasmic fraction lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear fraction lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 56 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467)

Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling KIAA0652/ATG13 with ab201467 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467)

KIAA0652/ATG13 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab201467 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab201467 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: HeLa whole cell lysate 10µg (Input).

Lane 2: ab201467 IP in HeLa whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab201467 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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