Anti-KIAA0652/ATG13 antibody (ab105392)
Key features and details
- Rabbit polyclonal to KIAA0652/ATG13
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-KIAA0652/ATG13 antibody
See all KIAA0652/ATG13 primary antibodies -
Description
Rabbit polyclonal to KIAA0652/ATG13 -
Host species
Rabbit -
Specificity
ab105392 is predicted to not cross-react with other ATG members. Two isoforms of KIAA0652/ATG13 are known to exist; this antibody will recognize both isoforms.
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Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human KIAA0652/ATG13 (C terminal).
Database link: NP_001136145 -
Positive control
- WB: Rat heart tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KIAA0652/ATG13 antibody (ab105392)All lanes : Anti-KIAA0652/ATG13 antibody (ab105392) at 1 µg/ml
Lane 1 : Rat heart tissue lysate without blocking peptide
Lane 2 : Rat heart tissue lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 57 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIAA0652/ATG13 antibody (ab105392)Immunohistochemistry of KIAA0652/ATG13 in Mouse heart, using ab105392 at a dilution of 5 µg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-KIAA0652/ATG13 antibody (ab105392)ICC/IF image of ab105392 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105392, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry/ Immunofluorescence - Anti-KIAA0652/ATG13 antibody (ab105392)Immunofluorescence of ATG13 in Mouse Heart cells using ab105392 at 20 ug/ml.
