Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Friday March 12, 2021

Anti-ANO6 antibody [EPR20910-105] (ab234422)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20910-105] to ANO6
Suitable for: WB, IP
Reacts with: Mouse, Rat

Product name

Anti-ANO6 antibody [EPR20910-105]
See all ANO6 primary antibodies
Description

Rabbit monoclonal [EPR20910-105] to ANO6
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Mouse

WB
Mouse

Rat

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IP: Mouse spleen lysate. WB: C6, NIH/3T3 whole cell lysate. Mouse and rat spleen tissue lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Mouse
WB	Mouse Rat

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20910-105
Isotype

IgG
Research areas

Western blot - Anti-ANO6 antibody [EPR20910-105] (ab234422)

All lanes : Anti-ANO6 antibody [EPR20910-105] (ab234422) at 1/1000 dilution

Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Rat spleen tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 106 kDa
Observed band size: 120-250 kDa
why is the actual band size different from the predicted?

The molecular weight observed is consistent with literatures (PMID: 29507235, PMID: 26417084, PMID: 27227820, PMID: 24478309).

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lanes 1-2: 37 secs; Lane 3: 124 secs; Lane 4: 70 secs.
Immunoprecipitation - Anti-ANO6 antibody [EPR20910-105] (ab234422)

ANO6 was immunoprecipitated from 0.35 mg mouse spleen lysate with ab234422 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab234422 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
Lane 1: Mouse spleen lysate 10 µg (Input).
Lane 2: ab234422 IP in mouse spleen lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab234422 in mouse spleen lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 mins.

The molecular weight observed is consistent with literatures (PMID:29507235, PMID: 26417084, PMID: 27227820, PMID: 24478309)
Anti-ANO6 antibody [EPR20910-105] (ab234422)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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