Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ANO6 antibody [EPR20910-105] (ab234422)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20910-105] to ANO6
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-ANO6 antibody [EPR20910-105]
    See all ANO6 primary antibodies

  • Description

    Rabbit monoclonal [EPR20910-105] to ANO6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: Mouse spleen lysate. WB: C6, NIH/3T3 whole cell lysate. Mouse and rat spleen tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20910-105

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ANO6 antibody [EPR20910-105] (ab234422)
    Western blot - Anti-ANO6 antibody [EPR20910-105] (ab234422)
    All lanes : Anti-ANO6 antibody [EPR20910-105] (ab234422) at 1/1000 dilution

    Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 3 : Mouse spleen tissue lysate
    Lane 4 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 106 kDa
    Observed band size: 120-250 kDa
    why is the actual band size different from the predicted?



    The molecular weight observed is consistent with literatures (PMID: 29507235, PMID: 26417084, PMID: 27227820, PMID: 24478309).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 37 secs; Lane 3: 124 secs; Lane 4: 70 secs.

  • Immunoprecipitation - Anti-ANO6 antibody [EPR20910-105] (ab234422)
    Immunoprecipitation - Anti-ANO6 antibody [EPR20910-105] (ab234422)

    ANO6 was immunoprecipitated from 0.35 mg mouse spleen lysate with ab234422 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab234422 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
    Lane 1: Mouse spleen lysate 10 µg (Input).
    Lane 2: ab234422 IP in mouse spleen lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab234422 in mouse spleen lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 mins.

    The molecular weight observed is consistent with literatures (PMID:29507235, PMID: 26417084, PMID: 27227820, PMID: 24478309)

  • Anti-ANO6 antibody [EPR20910-105] (ab234422)
    Anti-ANO6 antibody [EPR20910-105] (ab234422)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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