Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CRLF2 antibody [EPR22478-133] (ab224196)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22478-133] to CRLF2
  • Suitable for: Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CRLF2 antibody [EPR22478-133]
    See all CRLF2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22478-133] to CRLF2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: Human PBMCs.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22478-133

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-CRLF2 antibody [EPR22478-133] (ab224196)
    Flow Cytometry - Anti-CRLF2 antibody [EPR22478-133] (ab224196)

    Flow cytometric analysis of human peripheral blood mononuclear cells labeling CRLF2 with ab224196 at 1/60 dilution (Right compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left).

    Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or ab224196 (Right). Then stained with anti-CD11c conjugated to Alexa Fluor® 647.

    Gated on viable cells.

     

  • Flow Cytometry - Anti-CRLF2 antibody [EPR22478-133] (ab224196)
    Flow Cytometry - Anti-CRLF2 antibody [EPR22478-133] (ab224196)

    Flow cytometric analysis of human peripheral blood mononuclear cells labeling CRLF2 with ab224196 at 1/60 dilution (Right compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left).

    Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or ab224196 (Right). Then stained with anti-CD14 conjugated to Alexa Fluor® 647

    Gated on viable cells.

  • Flow Cytometry - Anti-CRLF2 antibody [EPR22478-133] (ab224196)
    Flow Cytometry - Anti-CRLF2 antibody [EPR22478-133] (ab224196)

    Flow cytometric analysis of LNCaP (human prostate cancer cell line) cells labeling CRLF2 with abX224196 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue).

    Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

    Negative control: LNCaP (PMID: 20236697). Gated on viable cells.

  • Anti-CRLF2 antibody [EPR22478-133] (ab224196)
    Anti-CRLF2 antibody [EPR22478-133] (ab224196)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CRLF2 antibody [EPR22478-133] (ab224196)

© 2021 Astana Biomed Group LLP. All rights reserved.