Creatine Assay Kit (Colorimetric/Fluorometric) (ab65339)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Urine
- Sensitivity: 0.001 mM
Overview
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Product name
Creatine Assay Kit (Colorimetric/Fluorometric) -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids -
Assay type
Quantitative -
Sensitivity
> 0.001 mM -
Range
0.001 mM - 10 mM -
Assay time
1h 00m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Creatine Assay Kit (Colorimetric/Fluorometric) (ab65339) provides an accurate, convenient measure of creatine in a variety of biological samples.
In the creatine assay protocol, creatine is enzymatically converted to sarcosine which is then specifically oxidized to generate a product that converts a colorless probe to an intensely red color (λmax = 570 nm), and highly fluorescent (Ex/Em = 538/587 nm) product. Creatine is therefore easily detected by either colorimetric or fluorometric methods. Detection range 0.001–10 mM Creatine.
Creatine assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 60 min
- analyze with a microplate reader -
Notes
Creatine is an endogenous compound whose function is to maintain a high ATP/ADP ratio, by way of its phosphorylated form and creatine kinase. Creatine supplementation has been used in the treatment of muscular, neurological and neurodegenerative diseases, as well as a sport performance enhancer. Detection of creatine level has wide applications in research and development.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Creatinase (Lyophilized) Blue 1 vial Creatine Assay Buffer WM, 1 x 25ml Creatine Enzyme Mix (Lyophilized) Green 1 vial Creatine Probe (in DMSO) Red 1 x 200µl Creatine Standard (10 µmol) (Lyophilized) Yellow 1 vial -
Research areas
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Relevance
Creatine is an endogenous amino acid that occurs in vertebrate tissues and in urine. In muscle tissue, creatine is generally found as its phosphorylated form phosphocreatine. Creatine functions as part of the cell's energy shuttle: the high energy phosphate group of ATP is transferred to creatinine to form phosphocreatinine in the following reaction: Cr + ATP ? P-Cr + ADP. This reaction is reversibly catalized by creatine kinase. In the human body, creatine is synthesized mainly in the liver from arginine, glycine and methionine. Creatine is excreted as creatinine in the urine.
Images
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Functional Studies - Creatine Assay Kit (ab65339)
Creatine measured in cell lysates showing quantity (nmol) per 106 cells
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Functional Studies - Creatine Assay Kit (ab65339)Creatine measured in biologicals showing concentration (micromolar)