Anti-CRLF2 antibody [EPR22478-133] - BSA and Azide free (ab256305)
![Anti-CRLF2 antibody [EPR22478-133] - BSA and Azide free (ab256305)](https://www.abcam.com/ps/products/256/ab256305/Images/ab256305-2-anti-crlf2-antibody-epr22478-133-flowcytometry-pbmc-cd11c-human.jpg "Anti-CRLF2 antibody [EPR22478-133] - BSA and Azide free (ab256305)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22478-133] to CRLF2 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CRLF2 antibody [EPR22478-133] - BSA and Azide free
See all CRLF2 primary antibodies -
Description
Rabbit monoclonal [EPR22478-133] to CRLF2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Human PBMCs.
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General notes
Ab256305 is the carrier-free version of ab224196. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab256305 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22478-133 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-CRLF2 antibody [EPR22478-133] - BSA and Azide free (ab256305)
Flow cytometric analysis of human peripheral blood mononuclear cells labeling CRLF2 with ab224196 at 1/60 dilution (Right compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left).
Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab224196 (Right). Then stained with anti-CD11c conjugated to Alexa Fluor® 647.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224196).
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![Flow Cytometry - Anti-CRLF2 antibody [EPR22478-133] - BSA and Azide free (ab256305)](https://www.abcam.com/ps/products/256/ab256305/Images/ab256305-1-anti-crlf2-antibody-epr22478-133-flowcytometry-pbmc-cd14-human.jpg)
Flow Cytometry - Anti-CRLF2 antibody [EPR22478-133] - BSA and Azide free (ab256305)Flow cytometric analysis of human peripheral blood mononuclear cells labeling CRLF2 with ab224196 at 1/60 dilution (Right compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left).
Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab224196 (Right). Then stained with anti-CD14 conjugated to Alexa Fluor® 647.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224196).
-
![Flow Cytometry - Anti-CRLF2 antibody [EPR22478-133] - BSA and Azide free (ab256305)](https://www.abcam.com/ps/products/256/ab256305/Images/ab256305-3-anti-crlf2-antibody-epr22478-133-flowcytometry-lncap-human.jpg)
Flow Cytometry - Anti-CRLF2 antibody [EPR22478-133] - BSA and Azide free (ab256305)Flow cytometric analysis of LNCaP (human prostate cancer cell line) cells labeling CRLF2 with ab224196 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue).
Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.
Negative control: LNCaP (PMID: 20236697). Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224196).
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![Anti-CRLF2 antibody [EPR22478-133] - BSA and Azide free (ab256305)](https://www.abcam.com/ps/products/256/ab256305/Images/ab256305-4-benefits-of-recombinant-antibodies.png)
Anti-CRLF2 antibody [EPR22478-133] - BSA and Azide free (ab256305)
