Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Sortilin/NT3 antibody [CL6526] (ab243043)

Key features and details

  • Mouse monoclonal [CL6526] to Sortilin/NT3
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Sortilin/NT3 antibody [CL6526]
    See all Sortilin/NT3 primary antibodies

  • Description

    Mouse monoclonal [CL6526] to Sortilin/NT3

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human Sortilin/NT3 aa 600-750.
    Database link: Q99523

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human cerebral cortex, testis, fallopian tube tissues; Mouse cerebral cortex and hippocampus tissue. WB: RH-30 and SK-MEL-30 cell lysate. ICC/IF: RH-30 cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)
    Immunocytochemistry/ Immunofluorescence - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)

    Paraformaldehyde-fixed, Triton X-100 permeabilized RH-30 cells stained for Sortilin/NT3 (Green) using ab243043 at 10 µg/ml in ICC/IF analysis. Microtubules are visulized in red, nucleus in blue.

  • Western blot - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)
    Western blot - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)
    All lanes : Anti-Sortilin/NT3 antibody [CL6526] (ab243043) at 1 µg/ml

    Lane 1 : RH-30 (Human bone marrow metastasis cell line) cells transfected with control siRNA
    Lane 2 : RH-30 cells transfected with target specific siRNA probe #1
    Lane 3 : RH-30 cells transfected with target specific siRNA probe #2


    Remaining relative intensity is presented.

    Loading control: Anti-GAPDH

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)

    Paraffin-embedded mouse hippocampus tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.

  • Western blot - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)
    Western blot - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)
    Anti-Sortilin/NT3 antibody [CL6526] (ab243043) at 1 µg/ml + SK-MEL-30 cell lysate
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)

    Paraffin-embedded mouse cerebral cortex tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)

    Paraffin-embedded human fallopian tube tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)

    Paraffin-embedded human testis tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)

    Paraffin-embedded human cerebral cortex tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)

    Negative control:

    Paraffin-embedded human lymph node tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.

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