Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Sortilin/NT3 antibody [CL6526] (ab243043)

Key features and details

Mouse monoclonal [CL6526] to Sortilin/NT3
Suitable for: IHC-P, WB, ICC/IF
Reacts with: Mouse, Human
Isotype: IgG2b

Product name

Anti-Sortilin/NT3 antibody [CL6526]
See all Sortilin/NT3 primary antibodies
Description

Mouse monoclonal [CL6526] to Sortilin/NT3
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human Sortilin/NT3 aa 600-750.
Database link: Q99523

Run BLAST with Run BLAST with
Positive control
- IHC-P: Human cerebral cortex, testis, fallopian tube tissues; Mouse cerebral cortex and hippocampus tissue. WB: RH-30 and SK-MEL-30 cell lysate. ICC/IF: RH-30 cells.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

CL6526
Isotype

IgG2b
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)

Paraformaldehyde-fixed, Triton X-100 permeabilized RH-30 cells stained for Sortilin/NT3 (Green) using ab243043 at 10 µg/ml in ICC/IF analysis. Microtubules are visulized in red, nucleus in blue.
Western blot - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)

All lanes : Anti-Sortilin/NT3 antibody [CL6526] (ab243043) at 1 µg/ml

Lane 1 : RH-30 (Human bone marrow metastasis cell line) cells transfected with control siRNA
Lane 2 : RH-30 cells transfected with target specific siRNA probe #1
Lane 3 : RH-30 cells transfected with target specific siRNA probe #2

Remaining relative intensity is presented.

Loading control: Anti-GAPDH
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)

Paraffin-embedded mouse hippocampus tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.
Western blot - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)

Anti-Sortilin/NT3 antibody [CL6526] (ab243043) at 1 µg/ml + SK-MEL-30 cell lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)

Paraffin-embedded mouse cerebral cortex tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)

Paraffin-embedded human fallopian tube tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)

Paraffin-embedded human testis tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)

Paraffin-embedded human cerebral cortex tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)

Negative control:

Paraffin-embedded human lymph node tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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