Anti-ANO6 antibody [EPR20910-105] - BSA and Azide free (ab256302)
![Anti-ANO6 antibody [EPR20910-105] - BSA and Azide free (ab256302)](https://www.abcam.com/ps/products/256/ab256302/Images/ab256302-1-anti-ano6-antibody-epr20910-105-immunoprecipitation-spleen-mouse.jpg "Anti-ANO6 antibody [EPR20910-105] - BSA and Azide free (ab256302)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20910-105] to ANO6 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-ANO6 antibody [EPR20910-105] - BSA and Azide free
See all ANO6 primary antibodies -
Description
Rabbit monoclonal [EPR20910-105] to ANO6 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: Mouse spleen lysate. WB: C6, NIH/3T3 whole cell lysate. Mouse and rat spleen tissue lysate.
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General notes
Ab256302 is the carrier-free version of ab234422. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab256302 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20910-105 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-ANO6 antibody [EPR20910-105] - BSA and Azide free (ab256302)
ANO6 was immunoprecipitated from 0.35 mg mouse spleen lysate with ab234422 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab234422 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
Lane 1: Mouse spleen lysate 10 µg (Input).
Lane 2: ab234422 IP in mouse spleen lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab234422 in mouse spleen lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 mins.The molecular weight observed is consistent with literatures (PMID:29507235, PMID: 26417084, PMID: 27227820, PMID: 24478309)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234422).
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![Anti-ANO6 antibody [EPR20910-105] - BSA and Azide free (ab256302)](https://www.abcam.com/ps/products/256/ab256302/Images/ab256302-2-benefits-of-recombinant-antibodies.png)
Anti-ANO6 antibody [EPR20910-105] - BSA and Azide free (ab256302)
