Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)
![Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)](https://www.abcam.com/ps/products/271/ab271851/Images/ab271851-3-ab52968240639antimertkantibodyy323immunoprecipitation.jpg "Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y323] to MERTK - BSA and Azide free
- Suitable for: IHC-P, WB, IP
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-MERTK antibody [Y323] - BSA and Azide free
See all MERTK primary antibodies -
Description
Rabbit monoclonal [Y323] to MERTK - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human tonsil and lymphoma tissues. IP: MERTK IP in HEK-293 cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
ab271851 is the carrier-free version of ab52968. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y323 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)
Lane 1: ab52968 (purified) at 1/20 immunoprecipitating MERTK in HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate.
Lane 2 - PBS.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52968).
Blocking/Dilution buffer and concentration: 5% NFDM/TBST. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)](https://www.abcam.com/ps/products/271/ab271851/Images/ab271851-2-ab52968240638antimertkantibodyy323immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling MERTK with purified ab52968 at 1/500.
Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Counterstained with hematoxylin.
Negative control using PBS instead of primary antibody (Inset).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52968). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)](https://www.abcam.com/ps/products/271/ab271851/Images/ab271851-1-ab5296865527antimertkantibodyy323immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma tissue labeling MERTK with unpurified ab52968 at a 1/50 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52968). -
![Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)](https://www.abcam.com/ps/products/271/ab271851/Images/ab271851-4-benefits-of-recombinant-antibodies.png)
Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)
