Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MERTK antibody [EPR17534-139] - BSA and Azide free (ab250715)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17534-139] to MERTK - BSA and Azide free
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-MERTK antibody [EPR17534-139] - BSA and Azide free
    See all MERTK primary antibodies

  • Description

    Rabbit monoclonal [EPR17534-139] to MERTK - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250715 is the carrier-free version of ab184086. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250715 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-MERTK antibody [EPR17534-139] - BSA and Azide free (ab250715)
    Western blot - Anti-MERTK antibody [EPR17534-139] - BSA and Azide free (ab250715)
    All lanes : Anti-MERTK antibody [EPR17534-139] (ab184086) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : Mouse liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 110 kDa
    Observed band size: 110-140,180-210 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab184086, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 17047157).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [EPR17534-139] - BSA and Azide free (ab250715)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [EPR17534-139] - BSA and Azide free (ab250715)
    This data was developed using ab184086, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling MERTK with ab184086 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membranous staining on mouse spleen (PMID: 19631584) is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [EPR17534-139] - BSA and Azide free (ab250715)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [EPR17534-139] - BSA and Azide free (ab250715)
    This data was developed using ab184086, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling MERTK with ab184086 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on hepatic sinusoids of mouse liver (PMID: 23799121) is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-MERTK antibody [EPR17534-139] - BSA and Azide free (ab250715)
    Immunoprecipitation - Anti-MERTK antibody [EPR17534-139] - BSA and Azide free (ab250715)

    This data was developed using ab184086, the same antibody clone in a different buffer formulation.

    MERTK was immunoprecipitated from 10 µg of mouse spleen tissue lysate with ab184086 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184086 at 1/1000 dilution. Secondary used is VeriBlot for IP Detection Reagent (HRP) (ab131366) at a 1/10,000 dilution.

    Lane 1: Mouse spleen tissue lysate, 10 µg (input).

    Lane 2: ab184086 IP in mouse spleen tissue lysate (+)

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184086 in mouse spleen lysate.

     b Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 5 seconds

    The molecular masses observed are consistent with what has been described in the literature. The band at approximately 50 kDa likely represents a cleavage fragment (PMID: 17047157, 15673687).

  • Immunoprecipitation - Anti-MERTK antibody [EPR17534-139] - BSA and Azide free (ab250715)
    Immunoprecipitation - Anti-MERTK antibody [EPR17534-139] - BSA and Azide free (ab250715)

    This data was developed using ab184086, the same antibody clone in a different buffer formulation.

    MERTK was immunoprecipitated from 10 µg of mouse spleen tissue lysate with ab184086 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184086 at 1/1000 dilution. Secondary used is VeriBlot for IP Detection Reagent (HRP) (ab131366) at a 1/10,000 dilution.

      Lane 1: Mouse spleen tissue lysate, 10 µg (input).

    Lane 2: ab184086 IP in mouse spleen tissue lysate (+)

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184086 in mouse spleen lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 5 seconds.

     The molecular masses observed are consistent with what has been described in the literature. The band at approximately 50 kDa likely represents a cleavage fragment (PMID: 17047157, 15673687).

  • Anti-MERTK antibody [EPR17534-139] - BSA and Azide free (ab250715)
    Anti-MERTK antibody [EPR17534-139] - BSA and Azide free (ab250715)

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