Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HNRNPA0 antibody [EP16085] - BSA and Azide free (ab236121)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP16085] to HNRNPA0 - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HNRNPA0 antibody [EP16085] - BSA and Azide free
    See all HNRNPA0 primary antibodies

  • Description

    Rabbit monoclonal [EP16085] to HNRNPA0 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human cerebral cortex tissue; Mouse spleen tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells. WB: HEK293T and A549 cell lysates.

  • General notes

    Ab236121 is the carrier-free version of ab197023. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236121 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-HNRNPA0 antibody [EP16085] - BSA and Azide free (ab236121)
    Western blot - Anti-HNRNPA0 antibody [EP16085] - BSA and Azide free (ab236121)
    All lanes : Anti-HNRNPA0 antibody [EP16085] (ab197023) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : HNRNPA0 knockout HEK293T cell lysate
    Lane 3 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 31 kDa
    Observed band size: 32,34 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab197023).

    Lanes 1-3: Merged signal (red and green). Green - ab197023 observed at 32,34 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab197023 Anti-HNRNPA0 antibody [EP16085]  was shown to specifically react with HNRNPA0 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266314 (knockout cell lysate ab257989) was used. Wild-type and HNRNPA0 knockout samples were subjected to SDS-PAGE. ab197023 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-HNRNPA0 antibody [EP16085] - BSA and Azide free (ab236121)
    Immunocytochemistry/ Immunofluorescence - Anti-HNRNPA0 antibody [EP16085] - BSA and Azide free (ab236121)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling HNRNPA0 with ab197023 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    -ve control 1: ab197023 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197023).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRNPA0 antibody [EP16085] - BSA and Azide free (ab236121)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRNPA0 antibody [EP16085] - BSA and Azide free (ab236121)

    Immunohistochemical analysis of paraffin-embedded Human cerebral cortex labeling HNRNPA0 with ab197023 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197023).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRNPA0 antibody [EP16085] - BSA and Azide free (ab236121)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRNPA0 antibody [EP16085] - BSA and Azide free (ab236121)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen labeling HNRNPA0 with ab197023 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Mouse spleen tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197023).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-HNRNPA0 antibody [EP16085] - BSA and Azide free (ab236121)
    Flow Cytometry - Anti-HNRNPA0 antibody [EP16085] - BSA and Azide free (ab236121)

    Flow cytometry analysis of HeLa cells labelling HNRNPA0 (red) with purified ab197023 at dilution of 1/150. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197023).

  • Anti-HNRNPA0 antibody [EP16085] - BSA and Azide free (ab236121)
    Anti-HNRNPA0 antibody [EP16085] - BSA and Azide free (ab236121)

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