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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules

Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] - BSA and Azide free (ab249262)

Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] - BSA and Azide free (ab249262)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11222] to Dynein intermediate chain 1/DNAI1 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] - BSA and Azide free
    See all Dynein intermediate chain 1/DNAI1 primary antibodies
  • Description

    Rabbit monoclonal [EPR11222] to Dynein intermediate chain 1/DNAI1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Dynein intermediate chain 1/DNAI1. The exact sequence is proprietary.
    Database link: O14576

  • General notes

    ab249262 is the carrier-free version of ab156567 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab249262 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Dynein intermediate chain 1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11222
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Dynein
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker

Images

  • Western blot - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] - BSA and Azide free (ab249262)
    Western blot - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] - BSA and Azide free (ab249262)
    All lanes : Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] (ab156567) at 1/1000 dilution

    Lane 1 : Fetal brain lysate
    Lane 2 : Human hypothalamus lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 79 kDa
    Observed band size: 74 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab156567, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] - BSA and Azide free (ab249262)
    Immunoprecipitation - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] - BSA and Azide free (ab249262)
    This data was developed using ab156567, the same antibody clone in a different buffer formulation.Immunoprecipitation. ab156567 at 1/1000 staining Dynein intermediate chain 1/DNAI1 in human fetal brain lysate immunoprecipitated using ab156567 at 1/10. Predicted band size: 74 kDa.
  • Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] - BSA and Azide free (ab249262)
    Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] - BSA and Azide free (ab249262)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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