Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

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Anti-MERTK antibody [EPR23248-259] (ab270448)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23248-259] to MERTK
Suitable for: WB
Reacts with: Mouse, Rat, Human

Product name

Anti-MERTK antibody [EPR23248-259]
See all MERTK primary antibodies
Description

Rabbit monoclonal [EPR23248-259] to MERTK
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293, HL-60, HepG2, and U-937 whole cell lysates. Human ovary, Human testis, Mouse spleen, Mouse kidney, Rat spleen, and Rat kidney tissue lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23248-259
Isotype

IgG
Research areas

Western blot - Anti-MERTK antibody [EPR23248-259] (ab270448)

All lanes : Anti-MERTK antibody [EPR23248-259] (ab270448) at 1/1000 dilution

Lane 1 : Human ovary tissue lysate
Lane 2 : Human testis tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 110 kDa
Observed band size: 150, 210 kDa
why is the actual band size different from the predicted?

Exposure time:

Lane 1: 3 minutes
Lane 2: 70 seconds

Blocking/Diluting buffer: 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.
Western blot - Anti-MERTK antibody [EPR23248-259] (ab270448)

All lanes : Anti-MERTK antibody [EPR23248-259] (ab270448) at 1/1000 dilution

Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Rat spleen tissue lysate
Lane 4 : Rat kidney tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 110 kDa
Observed band size: 210/150 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

Exposure time:

Lane 1: 70 seconds

Lanes 2-4: 3 minutes
Western blot - Anti-MERTK antibody [EPR23248-259] (ab270448)

All lanes : Anti-MERTK antibody [EPR23248-259] (ab270448) at 1/1000 dilution

Lane 1 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 4 : U-937 (human histiocytic lymphoma monocyte) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 110 kDa
Observed band size: 210/150 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Negative control: HL-60 (PMID:23474756)

This blot was developed using a higher sensitivity ECL substrate.

Exposure time: 48 seconds
Anti-MERTK antibody [EPR23248-259] (ab270448)

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