Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (ab176887)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y323] to MERTK - Low endotoxin, Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-MERTK antibody [Y323] - Low endotoxin, Azide free
    See all MERTK primary antibodies

  • Description

    Rabbit monoclonal [Y323] to MERTK - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab176887 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunoprecipitation - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (ab176887)
    Immunoprecipitation - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (ab176887)

    Lane 1: ab52968 (purified) at 1/20 immunoprecipitating MERTK in HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate.

    Lane 2 - PBS.

    For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52968).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (ab176887)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (ab176887)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling MERTK with purified ab52968 at 1/500.

    Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Counterstained with hematoxylin.

    Negative control using PBS instead of primary antibody (Inset).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52968).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (ab176887)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (ab176887)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma tissue labeling MERTK with unpurified ab52968 at a 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52968).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (ab176887)
    Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (ab176887)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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