Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MERTK antibody [EPR17534-139] (ab184086)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17534-139] to MERTK
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-MERTK antibody [EPR17534-139]
    See all MERTK primary antibodies

  • Description

    Rabbit monoclonal [EPR17534-139] to MERTK

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NIH/3T3 cell lysate; Mouse spleen and liver tissue lysate. IHC-P: Mouse liver and spleen tissue. IP: Mouse spleen tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MERTK antibody [EPR17534-139] (ab184086)
    Western blot - Anti-MERTK antibody [EPR17534-139] (ab184086)
    All lanes : Anti-MERTK antibody [EPR17534-139] (ab184086) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : Mouse liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 110 kDa
    Observed band size: 110-140,180-210 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    The expression profile observed is consistent with what has been described in the literature (PMID: 17047157).

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [EPR17534-139] (ab184086)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [EPR17534-139] (ab184086)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling MERTK with ab184086 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membranous staining on mouse spleen (PMID: 19631584) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [EPR17534-139] (ab184086)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [EPR17534-139] (ab184086)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling MERTK with ab184086 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on hepatic sinusoids of mouse liver (PMID: 23799121) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-MERTK antibody [EPR17534-139] (ab184086)
    Immunoprecipitation - Anti-MERTK antibody [EPR17534-139] (ab184086)

    MERTK was immunoprecipitated from 10 μg of mouse spleen tissue lysate with ab184086 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184086 at 1/1000 dilution. Secondary used is VeriBlot for IP Detection Reagent (HRP) (ab131366) at a 1/10,000 dilution.

    Lane 1: Mouse spleen tissue lysate, 10 μg (input).
    Lane 2: ab184086 IP in mouse spleen tissue lysate (+)
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184086 in mouse spleen lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST
    Exposure time: 5 seconds

    The molecular masses observed are consistent with what has been described in the literature. The band at approximately 50 kDa likely represents a cleavage fragment (PMID: 17047157, 15673687).

  • Anti-MERTK antibody [EPR17534-139] (ab184086)
    Anti-MERTK antibody [EPR17534-139] (ab184086)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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