Anti-GAB1 antibody (ab59362)
Key features and details
- Rabbit polyclonal to GAB1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GAB1 antibody
See all GAB1 primary antibodies -
Description
Rabbit polyclonal to GAB1 -
Host species
Rabbit -
Specificity
ab59362 detects endogenous levels of total GAB1 protein -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthesized non phosphopeptide derived from human GAB1 around the phosphorylation site of tyrosine 659 (VDYPVV).
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General notes
Store at -20°C for 1 yearReproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit antiserum by affinity chromatography, using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GAB1 antibody (ab59362)All lanes : Anti-GAB1 antibody (ab59362) at 1/500 dilution
Lane 1 : Extracts from NIH/3T3 cells with no immunizing peptide
Lane 2 : Extracts from NIH/3T3 cells with immunizing peptide
Observed band size: 77 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAB1 antibody (ab59362)ab59362, at a 1/50 dilution, staining human GAB1 in breast carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence of the immunizing peptide (left image) and in the presence of the immunizing peptide (right image). -
Immunocytochemistry/ Immunofluorescence - Anti-GAB1 antibody (ab59362)ICC/IF image of ab59362 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59362, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
