Anti-GAB1 (phospho Y627) antibody (ab131458)
Key features and details
- Rabbit polyclonal to GAB1 (phospho Y627)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-GAB1 (phospho Y627) antibody
See all GAB1 primary antibodies -
Description
Rabbit polyclonal to GAB1 (phospho Y627) -
Host species
Rabbit -
Specificity
ab131458 detects endogenous levels of GAB1 only when phosphorylated at Tyrosine 627. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Synthetic peptide from a region surrounding the phosphorylation site of Tyrosine 627 (
V-E-Y(p)L-D
) of Human GAB1 (NP_002030.2). -
Positive control
- Methanol fixed HeLa cells; HUVEC cell extracts EGF treated.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were purified by using epitope specific phospho peptide. Non-phospho specific antibodies were removed by chromatography using non-phospho peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-GAB1 (phospho Y627) antibody (ab131458)Immunofluorescence staining of methanol fixed HeLa cells labelling GAB1 (phospho Y627) with ab131458 antibody at a dilution of 1/100.
-
Western blot - Anti-GAB1 (phospho Y627) antibody (ab131458)All lanes : Anti-GAB1 (phospho Y627) antibody (ab131458) at 1/500 dilution
Lane 1 : HUVEC cell extracts untreated
Lane 2 : HUVEC cell extracts EGF treated
Predicted band size: 77 kDa
