Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GAB1 antibody [EPR375] (ab133486)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR375] to GAB1
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-GAB1 antibody [EPR375]
    See all GAB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR375] to GAB1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human GAB1 aa 500-600. The exact sequence is proprietary.
    Database link: Q13480

  • Positive control

    • WB: HepG2, K562, 293T, and SH-SY5Y cell lysates

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-GAB1 antibody [EPR375] (ab133486)
    Western blot - Anti-GAB1 antibody [EPR375] (ab133486)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: GAB1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: Hek293 whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab133486 observed at 100 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab133486 was shown to recognize GAB1 in wild-type HAP1 cells as signal was lost at the expected MW in GAB1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GAB1 knockout samples were subjected to SDS-PAGE. Ab133486 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-GAB1 antibody [EPR375] (ab133486)
    Western blot - Anti-GAB1 antibody [EPR375] (ab133486)
    Anti-GAB1 antibody [EPR375] (ab133486) at 1/2000 dilution (purified) + K562 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 77 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-GAB1 antibody [EPR375] (ab133486)
    Western blot - Anti-GAB1 antibody [EPR375] (ab133486)
    Anti-GAB1 antibody [EPR375] (ab133486) at 1/2000 dilution (purified) + HEK293 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 77 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-GAB1 antibody [EPR375] (ab133486)
    Western blot - Anti-GAB1 antibody [EPR375] (ab133486)
    All lanes : Anti-GAB1 antibody [EPR375] (ab133486) at 1/1000 dilution (unpurified)

    Lane 1 : HepG2 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 77 kDa

  • Immunoprecipitation - Anti-GAB1 antibody [EPR375] (ab133486)
    Immunoprecipitation - Anti-GAB1 antibody [EPR375] (ab133486)

    ab133486 (purified) at 1/80 immunoprecipitating GAB1 in HEK293 whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/10,000) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-GAB1 antibody [EPR375] (ab133486)
    Anti-GAB1 antibody [EPR375] (ab133486)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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