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Anti-AK2 antibody [EPR11388(B)] (ab166901)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11388(B)] to AK2
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-AK2 antibody [EPR11388(B)]
    See all AK2 primary antibodies

  • Description

    Rabbit monoclonal [EPR11388(B)] to AK2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human AK2. The exact sequence is proprietary.

  • Positive control

    • WB: Fetal kidney, Fetal liver, HEK-293T, HL-60, HepG2, MCF-7 and HeLa lysates. IHC-P: Paraffin-embedded Human kidney tissue and paraffin-embedded Human stomach tissue. ICC: MCF7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-AK2 antibody [EPR11388(B)] (ab166901)
    Western blot - Anti-AK2 antibody [EPR11388(B)] (ab166901)
    All lanes : Anti-AK2 antibody [EPR11388(B)] (ab166901) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : AK2 knockout HEK-293T cell lysate
    Lane 3 : HL-60 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab166901 observed at 26 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

    ab166901 was shown to react with AK2 in wild-type HEK-293T cells in western blot with loss of signal observed in AK2 knockout cell line ab266539 (AK2 knockout cell lysate ab257825). Wild-type and AK2 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab166901 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11388(B)] (ab166901)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11388(B)] (ab166901)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling AK2 using ab166901 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-AK2 antibody [EPR11388(B)] (ab166901)
    Immunocytochemistry/ Immunofluorescence - Anti-AK2 antibody [EPR11388(B)] (ab166901)

    Immunocytochemistry/Immunofluorescence analysis MCF-7 (human breast carcinoma) cells labelling AK2 with purified ab166901 at a dilution of 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at dilution of 1/1000 was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • Western blot - Anti-AK2 antibody [EPR11388(B)] (ab166901)
    Western blot - Anti-AK2 antibody [EPR11388(B)] (ab166901)
    All lanes : Anti-AK2 antibody [EPR11388(B)] (ab166901) at 1/1000 dilution

    Lane 1 : Fetal kidney lysate
    Lane 2 : Fetal liver lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP antibody at 1/2000 dilution

    Predicted band size: 26 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11388(B)] (ab166901)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11388(B)] (ab166901)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling AK2 using ab166901 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-AK2 antibody [EPR11388(B)] (ab166901)
    Immunoprecipitation - Anti-AK2 antibody [EPR11388(B)] (ab166901)
    Immunoprecipitation of AK2 from HeLa cell lysate pellet using ab166901 at 1/10 dilution followed by immunoblotting. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
  • Anti-AK2 antibody [EPR11388(B)] (ab166901)
    Anti-AK2 antibody [EPR11388(B)] (ab166901)

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