Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rad51D antibody [EPR16205] (ab202063)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16205] to Rad51D
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Rad51D antibody [EPR16205]
    See all Rad51D primary antibodies

  • Description

    Rabbit monoclonal [EPR16205] to Rad51D

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    Human
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293, HeLa, Jurkat and C6 cell lysates; Rat kidney lysate. IHC-P: Human lung carcinoma, Human tonsil, Human small intestine and Rat kidney tissues. ICC/IF: A549 and HeLa cells. IP: HeLa whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16205

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Rad51D antibody [EPR16205] (ab202063)
    Western blot - Anti-Rad51D antibody [EPR16205] (ab202063)
    All lanes : Anti-Rad51D antibody [EPR16205] (ab202063) at 1/1000 dilution

    Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 35 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Rad51D antibody [EPR16205] (ab202063)
    Western blot - Anti-Rad51D antibody [EPR16205] (ab202063)
    All lanes : Anti-Rad51D antibody [EPR16205] (ab202063) at 1/1000 dilution

    Lane 1 : Rat kidney lysate
    Lane 2 : C6 (Rat glial tumor cells) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 35 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] (ab202063)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] (ab202063)

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling Rad51D with ab202063 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on Human lung carcinoma tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] (ab202063)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] (ab202063)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Rad51D with ab202063 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Nuclear staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] (ab202063)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] (ab202063)

    Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling Rad51D with ab202063 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Nuclear staining on Human small intestine tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] (ab202063)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] (ab202063)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Rad51D with ab202063 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Nuclear staining on rat kidney tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Rad51D antibody [EPR16205] (ab202063)
    Immunoprecipitation - Anti-Rad51D antibody [EPR16205] (ab202063)

    Rad51D was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab202063 at 1/50 dilution.

    Western blot was performed from the immunoprecipitate using ab202063 at 1/1000 dilution.

    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (Input).

    Lane 2: ab202063 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab202063 in HeLa whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Anti-Rad51D antibody [EPR16205] (ab202063)
    Anti-Rad51D antibody [EPR16205] (ab202063)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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