Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸ 10 µl 117 264 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Rhodopsin antibody [EPR21876] (ab221664)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21876] to Rhodopsin
Suitable for: WB, IHC-P, IHC-Fr, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-Rhodopsin antibody [EPR21876]
See all Rhodopsin primary antibodies
Description

Rabbit monoclonal [EPR21876] to Rhodopsin
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat Human
IP	Rat
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human, mouse and rat eye tissue lysates; Rat retina lysate. IHC-P: Human, mouse and rat retina tissues. IHC-Fr: Mouse and rat retina. IP: Rat eye tissue lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21876
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR21876] (ab221664)

Immunohistochemical analysis of paraffin-embedded human retina tissue labeling Rhodopsin with ab221664 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in outer segment of human retina is observed (PMID: 23223288). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-Rhodopsin antibody [EPR21876] (ab221664)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse retina tissue labeling Rhodopsin with ab221664 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining in the outer segment of mouse retina is observed (PMID: 21938483).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-Rhodopsin antibody [EPR21876] (ab221664)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat retina tissue labeling Rhodopsin with ab221664 at 1/500 dilution,followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining in the outer segment of rat retina is observed (PMID: 21938483).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR21876] (ab221664)

Immunohistochemical analysis of paraffin-embedded mouse retina tissue labeling Rhodopsin with ab221664 at 1/32000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in outer segment of mouse retina is observed (PMID: 23223288). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR21876] (ab221664)

Immunohistochemical analysis of paraffin-embedded rat retina tissue labeling Rhodopsin with ab221664 at 1/32000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in outer segment of rat retina is observed (PMID: 23223288). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Rhodopsin antibody [EPR21876] (ab221664)

Lanes 1-3 : Anti-Rhodopsin antibody [EPR21876] (ab221664) at 1/1000 dilution
Lane 4 : Anti-Rhodopsin antibody [EPR21876] (ab221664) at 1/5000 dilution

Lane 1 : Human eye tissue at 20 µg
Lane 2 : Mouse eye tissue at 20 µg
Lane 3 : Rat retina tissue lysate at 20 µg
Lane 4 : Rat eye tissue lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 38 kDa
Observed band size: 35-150 kDa
why is the actual band size different from the predicted?

Exposure Time: Lane 1: 3 minutes; Lane 2: 3 seconds; Lane 3: 58 seconds; Lane 4: 26 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

The multiple bands (>60 kDa) correspond to dimers and multimers of rhodopsin, consistent with the literature (PMID: 25270370; PMID: 22219383).
Immunoprecipitation - Anti-Rhodopsin antibody [EPR21876] (ab221664)

Rhodopsin was immunoprecipitated from 0.35 mg rat eye tissue lysate with ab221664 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221664 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

Lane 1: Rat eye tissue lysate 10 μg (input).

Lane 2: Rat eye tissue lysate (boiled) 10 μg (input).

Lane 3: ab221664 IP in Rat eye tissue lysate.

Lane 4: Rabbit monoclonal IgG (ab172730) instead of ab221664 in rat eye tissue lysate.

Exposure time: 15 seconds

The multiple bands (>60 kDa) correspond to dimers and multimers of rhodopsin, consistent with the literature (PMID: 25270370; PMID: 22219383).

We do not recommend boiling samples in loading buffer as this may cause protein aggregation (lane 2).
Anti-Rhodopsin antibody [EPR21876] (ab221664)

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