Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rhodopsin antibody [EPR21876] - BSA and Azide free (ab232934)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21876] to Rhodopsin - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Rhodopsin antibody [EPR21876] - BSA and Azide free
    See all Rhodopsin primary antibodies

  • Description

    Rabbit monoclonal [EPR21876] to Rhodopsin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human retina tissue.

  • General notes

    ab232934 is the carrier-free version of ab221664.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR21876] - BSA and Azide free (ab232934)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR21876] - BSA and Azide free (ab232934)

    Immunohistochemical analysis of paraffin-embedded rat retina tissue labeling Rhodopsin with ab221664 at 1/32000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in outer segment of rat retina is observed (PMID: 23223288). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221664).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Rhodopsin antibody [EPR21876] - BSA and Azide free (ab232934)
    Immunoprecipitation - Anti-Rhodopsin antibody [EPR21876] - BSA and Azide free (ab232934)

    Rhodopsin was immunoprecipitated from 0.35 mg rat eye tissue lysate with ab221664 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221664 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

    Lane 1: Rat eye tissue lysate 10 μg (input).

    Lane 2: Rat eye tissue lysate (boiled) 10 μg (input).

    Lane 3: ab221664 IP in Rat eye tissue lysate.

    Lane 4: Rabbit monoclonal IgG (ab172730) instead of ab221664 in rat eye tissue lysate.

    Exposure time: 15 seconds

    The multiple bands (>60 kDa) correspond to dimers and multimers of rhodopsin, consistent with the literature (PMID: 25270370; PMID: 22219383).

    We do not recommend boiling samples in loading buffer as this may cause protein aggregation (lane 2).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221664).

  • Immunohistochemistry (Frozen sections) - Anti-Rhodopsin antibody [EPR21876] - BSA and Azide free (ab232934)
    Immunohistochemistry (Frozen sections) - Anti-Rhodopsin antibody [EPR21876] - BSA and Azide free (ab232934)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat retina tissue labeling Rhodopsin with ab221664 at 1/500 dilution,followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining in the outer segment of rat retina is observed (PMID: 21938483).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221664).

  • Immunohistochemistry (Frozen sections) - Anti-Rhodopsin antibody [EPR21876] - BSA and Azide free (ab232934)
    Immunohistochemistry (Frozen sections) - Anti-Rhodopsin antibody [EPR21876] - BSA and Azide free (ab232934)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse retina tissue labeling Rhodopsin with ab221664 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining in the outer segment of mouse retina is observed (PMID: 21938483).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221664).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR21876] - BSA and Azide free (ab232934)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR21876] - BSA and Azide free (ab232934)

    Immunohistochemical analysis of paraffin-embedded mouse retina tissue labeling Rhodopsin with ab221664 at 1/32000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in outer segment of mouse retina is observed (PMID: 23223288). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221664).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR21876] - BSA and Azide free (ab232934)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [EPR21876] - BSA and Azide free (ab232934)

    Immunohistochemical analysis of paraffin-embedded human retina tissue labeling Rhodopsin with ab221664 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in outer segment of human retina is observed (PMID: 23223288). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221664).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Rhodopsin antibody [EPR21876] - BSA and Azide free (ab232934)
    Anti-Rhodopsin antibody [EPR21876] - BSA and Azide free (ab232934)

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