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Signal Transduction Signaling Pathway G Protein Signaling GPCR

Anti-Rhodopsin antibody [A531] (ab190307)

Price and availability

274 732 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-Rhodopsin antibody [A531] (ab190307)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [A531] to Rhodopsin
  • Suitable for: ICC/IF, WB
  • Reacts with: Cow, Pig
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Rhodopsin antibody [A531]
    See all Rhodopsin primary antibodies
  • Description

    Mouse monoclonal [A531] to Rhodopsin
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Cow, Pig
    Predicted to work with: Sheep, Rabbit, Cat, Dog, Cynomolgus monkey, Chinese hamster
  • Immunogen

    Full length native protein (purified) corresponding to Cow Rhodopsin aa 1 to the C-terminus.
    Database link: P02699

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Bovine retinal extracts; Pig retinal tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.03% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    A531
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Neuroscience
    • Sensory System
    • Visual system

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Rhodopsin antibody [A531] (ab190307)
    Immunocytochemistry/ Immunofluorescence - Anti-Rhodopsin antibody [A531] (ab190307)

    Immunofluorescent analysis of pig retinal tissue labeling Rhodopsin with ab190307 at 1/1000 dilution (green) and counterstained with rabbit polyclonal antibody to neurofilament  (red) and DNA (blue).

    Rhodopsin is most abundant in the outer segments of retina (OS), NF-M is abundant in the optic nerve fiber layer (ONFL), but seen in processes and neurons in other regions also. Other layers are pigmented epithelium (PE), outer and inner nuclear layers (ONL, INL), outer and inner plexiform layers (OPL, IPL) and ganglion cell layer (GCL).

  • Western blot - Anti-Rhodopsin antibody [A531] (ab190307)
    Western blot - Anti-Rhodopsin antibody [A531] (ab190307)
    Anti-Rhodopsin antibody [A531] (ab190307) at 1/5000 dilution + Bovine retinal extract

    Predicted band size: 39 kDa



    Bands about 70 kDa and 140 kDa are aggregated forms of Rhodopsin.

    Note, due to the highly hydrophobic nature of rhodopsin, it is important not to boil a sample containing it in SDS-PAGE sample buffer, as this will result in more extensive aggregation of the Rhodopsin protein.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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