Anti-Rhodopsin antibody [B630] (ab230692)
Key features and details
- Mouse monoclonal [B630] to Rhodopsin
- Suitable for: IHC-P, WB
- Reacts with: Cow, Human, Pig
- Isotype: IgG1
Overview
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Product name
Anti-Rhodopsin antibody [B630]
See all Rhodopsin primary antibodies -
Description
Mouse monoclonal [B630] to Rhodopsin -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanPigWB Cow -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- IHC-P: Human and pig retina tissue. WB: Bovine retinal extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.07% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Protein G purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
B630 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Formalin-fixed, paraffin-embedded human retinal tissue stained for Rhodopsin with ab230692 at 10 µg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded pig retinal tissue stained for Rhodopsin with ab230692 (green) at 10 µg/ml in immunohistochemical analysis.
Rhodopsin is most abundant in the rod outer segments (ROS) of retina, clearly localized in rod membranes. The rod inner segments (RIS) and rod nuclei in the outer nuclear layer (ONL) are also seen in this image. Nuclear DNA was stained with DAPI (blue).
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Anti-Rhodopsin antibody [B630] (ab230692) at 1/5000 dilution + bovine retinal extracts
Developed using the ECL technique.Bands about 70 kDa and 140 kDa are aggregated forms of rhodopsin.