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Signal Transduction Signaling Pathway G Protein Signaling GPCR

Anti-Rhodopsin antibody [B630] (ab230692)

Anti-Rhodopsin antibody [B630] (ab230692)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [B630] to Rhodopsin
  • Suitable for: IHC-P, WB
  • Reacts with: Cow, Human, Pig
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Rhodopsin antibody [B630]
    See all Rhodopsin primary antibodies
  • Description

    Mouse monoclonal [B630] to Rhodopsin
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    Pig
    WB
    Cow
    See all applications and species data
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • IHC-P: Human and pig retina tissue. WB: Bovine retinal extracts.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.07% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Protein G purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    B630
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Neuroscience
    • Sensory System
    • Visual system

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [B630] (ab230692)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [B630] (ab230692)

    Formalin-fixed, paraffin-embedded human retinal tissue stained for Rhodopsin with ab230692 at 10 µg/ml in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [B630] (ab230692)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [B630] (ab230692)

    Formalin-fixed, paraffin-embedded pig retinal tissue stained for Rhodopsin with ab230692 (green) at 10 µg/ml in immunohistochemical analysis.

    Rhodopsin is most abundant in the rod outer segments (ROS) of retina, clearly localized in rod membranes. The rod inner segments (RIS) and rod nuclei in the outer nuclear layer (ONL) are also seen in this image. Nuclear DNA was stained with DAPI (blue).

  • Western blot - Anti-Rhodopsin antibody [B630] (ab230692)
    Western blot - Anti-Rhodopsin antibody [B630] (ab230692)
    Anti-Rhodopsin antibody [B630] (ab230692) at 1/5000 dilution + bovine retinal extracts

    Developed using the ECL technique.


    Bands about 70 kDa and 140 kDa are aggregated forms of rhodopsin.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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