Antibodies, Proteins, Kits and Reagents for Life Science

341 740 ₸ Product size

100 µl 341 740 ₸ 10 µl 144 067 ₸

Order now and get it on Tuesday March 02, 2021

Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] (ab133607)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPNCIR152] to hnRNP C1 + C2/HNRNPC
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152]
See all hnRNP C1 + C2/HNRNPC primary antibodies
Description

Rabbit monoclonal [EPNCIR152] to hnRNP C1 + C2/HNRNPC
Host species

Rabbit
Specificity

This antibody is specific to isoform C2.

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

corresponding to Human hnRNP C1 + C2/HNRNPC aa 100-200.
Database link: P07910
Positive control
- WB: HeLa, MCF7, HepG2, K562, and 293T cell lysates IHC-P: Human kidney and Human thyroid carcinoma tissues
General notes
This antibody was developed as part of a collaboration between the National Cancer Institute's Center for Cancer Research and the lab of Mirit Aladjem. View antibodies from NCI Center for Cancer Research Collaboration.

This product was previously labelled as hnRNP C1 + C2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPNCIR152
Isotype

IgG
Research areas

Western blot - Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] (ab133607)

All lanes : Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] (ab133607) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : K562 cell lysate
Lane 5 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 33 kDa

Actual band size : 42 kDa
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] (ab133607)

Immunofluorescence staining of MCF-7 cells with purified ab133607 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor^®488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] (ab133607)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling hnRNP C1 + C2/HNRNPC with ab133607 antibody at a dilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] (ab133607)

Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labelling hnRNP C1 + C2/HNRNPC with ab133607 antibody at a dilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-hnRNP C1 + C2/HNRNPC antibody [EPNCIR152] (ab133607)

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