Anti-CTLA4 antibody [EPR21972-205] (ab224295)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21972-205] to CTLA4
- Suitable for: Flow Cyt
- Reacts with: Mouse
Overview
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Product name
Anti-CTLA4 antibody [EPR21972-205]
See all CTLA4 primary antibodies -
Description
Rabbit monoclonal [EPR21972-205] to CTLA4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Mouse primary splenocytes treated with ConA .
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21972-205 -
Isotype
IgG -
Research areas
Images
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Flow cytometric analysis of mouse primary splenocytes treated with ConA (2.5 μg/ml, 3 days) labeling CTLA4 with ab224295 at 1/100 dilution (Right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
A low level of CTLA4 is expected to be on the cell surface; the majority is intracellular, localized to secretory lysosomes (PMID: 11046036). Gated on viable cells.
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