Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CTLA4 antibody [CAL49] - BSA and Azide free (ab251599)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CAL49] to CTLA4 - BSA and Azide free
  • Suitable for: IHC-P, WB, Flow Cyt, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CTLA4 antibody [CAL49] - BSA and Azide free
    See all CTLA4 primary antibodies

  • Description

    Rabbit monoclonal [CAL49] to CTLA4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CTLA4 aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: P16410

  • Positive control

    • IHC-P: Human tonsil, lymph node and breast carcinoma tissues. WB: Human PBMCs (treated with 10µg/ml PHA for 2 days) whole cell lysate; mouse splenocytes (treated with 2.5µg/ml Concanavalin A (ConA) for 3 days) whole cell lysate. Flow cyt: Human PBMCs (treated with 10µg/ml PHA for 2 days); mouse splenocytes (treated with 2.5µg/ml Concanavalin A (ConA) for 3 days). IP: Human tonsil lysate.

  • General notes

    Ab251599 is the carrier-free version of ab237712. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251599 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTLA4 antibody [CAL49] - BSA and Azide free (ab251599)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTLA4 antibody [CAL49] - BSA and Azide free (ab251599)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CTLA4 with ab237712 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human tonsil is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

    The section was incubated with ab237712 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237712).

  • Flow Cytometry - Anti-CTLA4 antibody [CAL49] - BSA and Azide free (ab251599)
    Flow Cytometry - Anti-CTLA4 antibody [CAL49] - BSA and Azide free (ab251599)

    Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween 20 permeabilized mouse splenocytes (treated with 2.5μg/ml Concanavalin A (ConA) for 3 days) cells labeling CTLA4 with ab237712 at 1/400 (Right) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left).

    Cells were surface stained with anti-CD3 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA for 10min followed by intracellular staining with rabbit IgG (Left) and ab237712 (Right).

    Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237712).

  • Immunoprecipitation - Anti-CTLA4 antibody [CAL49] - BSA and Azide free (ab251599)
    Immunoprecipitation - Anti-CTLA4 antibody [CAL49] - BSA and Azide free (ab251599)

    CTLA4 was immunoprecipitated from 0.35 mh human tonsil lysate with ab237712 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab237712 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.

    Lane 1: Human tonsil lysate 10 μg (Input). 
    Lane 2: ab237712 IP in human tonsil lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237712 in human tonsil lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237712).

  • Flow Cytometry - Anti-CTLA4 antibody [CAL49] - BSA and Azide free (ab251599)
    Flow Cytometry - Anti-CTLA4 antibody [CAL49] - BSA and Azide free (ab251599)

    Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween 20 permeabilized human PBMC (peripheral blood mononuclear cell) (treated with 10μg/ml PHA for 2 days) cells labeling CTLA4 with ab237712 at 1/400 (Right) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left).

    Cells were surface stained with anti-CD3 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA for 10min followed by intracellular staining with rabbit IgG (Left) and ab237712 (Right).

    Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237712).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTLA4 antibody [CAL49] - BSA and Azide free (ab251599)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTLA4 antibody [CAL49] - BSA and Azide free (ab251599)

    Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for CTLA4 using ab237712 at 0.25 μg/ml in immunohistochemical analysis.

    Incubate with primary antibody for 75 minutes at room temperature.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237712).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTLA4 antibody [CAL49] - BSA and Azide free (ab251599)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTLA4 antibody [CAL49] - BSA and Azide free (ab251599)

    Formalin-fixed, paraffin-embedded human lymph node tissue stained for CTLA4 using ab237712 at 0.25 μg/ml in immunohistochemical analysis.

    Incubate with primary antibody for 75 minutes at room temperature.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237712).

  • Anti-CTLA4 antibody [CAL49] - BSA and Azide free (ab251599)
    Anti-CTLA4 antibody [CAL49] - BSA and Azide free (ab251599)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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