Anti-Hormone sensitive lipase/HSL antibody (ab45422)
Key features and details
- Rabbit polyclonal to Hormone sensitive lipase/HSL
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Hormone sensitive lipase/HSL antibody
See all Hormone sensitive lipase/HSL primary antibodies -
Description
Rabbit polyclonal to Hormone sensitive lipase/HSL -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Hormone sensitive lipase/HSL (C terminal).
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General notes
This product was previously labelled as Hormone sensitive lipase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody is an immunoaffinity purified IgG -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Hormone sensitive lipase/HSL antibody (ab45422) This image is courtesy of an Abreview submitted by Kirk McManus
ab45422 staining Hormone sensitive lipase/HSL in the HeLa cell line from Human cervical cancer by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 16 hour at 22°C. ab150081 an Alexa Fluor®488-conjugated Goat anti-rabbit IgG H&L (1/200) polyclonal was used as the secondary antibody. Nuclei were stained with DAPI.MeOH-fixed samples were also evaluated and failed to produce the expected staining pattern.
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Western blot - Anti-Hormone sensitive lipase/HSL antibody (ab45422)Lane 1 : Anti-Hormone sensitive lipase/HSL antibody (ab45422) at 0.5 µg/ml
Lane 2 : Anti-Hormone sensitive lipase/HSL antibody (ab45422) at 1 µg/ml
All lanes : Human lymph node tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 117 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa, 86 kDa, 88 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry/ Immunofluorescence - Anti-Hormone sensitive lipase/HSL antibody (ab45422)Immunofluorescence of Lipe in Human Lymph Node cells using ab45422 at 20 ug/ml.
