Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400)
![Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400)](https://www.abcam.com/ps/products/109/ab109400/Images/ab109400-122207-anti-hormone-sensitive-lipase-phospho-s853-antibody-epr23292-western-blot.jpg "Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2329(2)] to Hormone sensitive lipase/HSL (phospho S853)
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)]
See all Hormone sensitive lipase/HSL primary antibodies -
Description
Rabbit monoclonal [EPR2329(2)] to Hormone sensitive lipase/HSL (phospho S853) -
Host species
Rabbit -
Specificity
The antibody only detects HSL phosphorylated on serine 853. -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human skeletal muscle lysates and Mouse muscle lysates
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General notes
This product was previously labelled as Hormone sensitive lipase
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2329(2) -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Images
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Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400)All lanes : Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400) at 1/50000 dilution
Lane 1 : Human skeletal muscle lysates
Lane 2 : Human skeletal muscle lysates treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size: 117 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted?
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![Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400)](https://www.abcam.com/ps/products/109/ab109400/Images/ab109400-122206-anti-hormone-sensitive-lipase-phospho-s853-antibody-epr23292-western-blot.jpg)
Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400)All lanes : Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400) at 1/50000 dilution
Lane 1 : Mouse muscle lysate
Lane 2 : Mouse muscle lysate treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size: 117 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted?
-
![Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400)](https://www.abcam.com/ps/products/109/ab109400/Images/ab109400-3-benefits-of-recombinant-antibodies.png)
Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400)
