Protein A ELISA Kit (ab133036)
Key features and details
- Sensitivity: 3.35 pg/ml
- Range: 15.63 pg/ml - 1000 pg/ml
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
Overview
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Product name
Protein A ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Low 127.9pg/ml 5.2% Medium 364.3pg/ml 6.4% High 727.8pg/ml 5.5% Inter-assay Sample n Mean SD CV% Low 103.6pg/ml 13.4% Medium 209.7pg/ml 8.1% High 378.3pg/ml 8% -
Assay type
Sandwich (quantitative) -
Sensitivity
3.35 pg/ml -
Range
15.63 pg/ml - 1000 pg/ml -
Assay time
3h 00m -
Assay duration
Multiple steps standard assay -
Product overview
Abcam’s Protein A in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of natural and recombinant Protein A in neutralized buffers.
A chicken IgY antibody specific to Protein A has been precoated onto 96-well plates. Standards or test samples are added to the wells, incubated and then washed. A Protein A polyclonal antibody is then added, incubated and washed. An HRP conjugated antibody is then added incubated. The plate is washed once more and the TMB substrate is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound
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Notes
Staphylococcus aureus Protein A is a 42 kDa cell wall constituent that is characterized by its binding affinity to the Fc portion of some immunoglobulins, especially the IgG class. The IgG binding domain (domain B) consists of three anti-parallel alpha-helices, the third of which is disrupted when the protein is complexed with Fc. Protein A is commonly used to purify antisera and in commonly used immunodetection and visualization techniques. Protein A participates in a number of protective biological functions including anti-tumor, toxic and carcinogenic activities, thus necessitating the removal of potential Protein A contaminants from antibody preparations for therapeutic use.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 100ml Assay Buffer 13 1 x 55ml Chicken anti-Protein A Microplate (12 x 8 wells) 1 unit Plate Sealer 2 units Protein A Antibody 1 x 10ml Protein A HRP-Streptavidin Conjugate 1 x 10ml Protein A Standard 1 vial Stop Solution 2 1 x 10ml TMB Substrate 1 x 10ml -
Research areas
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Cellular localization
Secreted -
Alternative names
- Protein A
- SpA
Images
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Typical Standard Curve
Representative Standard Curve using ab133036.
