Protein A ELISA Kit (ab215542)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.7 pg/ml
- Range: 7.8 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
Overview
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Product name
Protein A ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 3.7% Inter-assay Sample n Mean SD CV% Overall 3 10.2% -
Sample type
Cell culture supernatant -
Assay type
Sandwich (quantitative) -
Sensitivity
3.7 pg/ml -
Range
7.8 pg/ml - 500 pg/ml -
Assay time
1h 30m -
Assay duration
One step assay -
Product overview
Protein A ELISA Kit (ab215542) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Protein A protein in cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Protein A with 3.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Protein A (Staphylococcus aureus Protein A) is a 42 kDa protein that is characterized by its ability to bind the Fc portion of immunoglobulins.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Protein A Capture Antibody 1 x 600µl 10X Protein A Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BC 1 x 6ml Plate Seals 1 unit Protein A Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. -
Cellular localization
Secreted; cell wall. -
Alternative names
- gG-binding protein A
- Immunoglobulin G binding protein A
- SPA
- Staphylococcal protein A
Images
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Other - Protein A ELISA Kit (ab215542)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Protein A standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of Protein A in Rabbit Monoclonal Antibody Protein A purified sample based on a 100 µg/mL antibody load.The concentrations of Protein A were measured in duplicate and interpolated from the Protein A standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Protein A concentration was determined to be 442 pg/mL in the Rabbit Monoclonal Antibody sample.
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MabSelect Protein A and natural native Protein A were measured in duplicate and interpolated from the recombinant Protein A standard curve and corrected for sample dilution.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
