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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Splicing

Anti-SNRPD3/Sm-D3 antibody [EPR7676] (ab128857)

Price and availability

241 228 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-SNRPD3/Sm-D3 antibody [EPR7676] (ab128857)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7676] to SNRPD3/Sm-D3
  • Suitable for: WB
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-SNRPD3/Sm-D3 antibody [EPR7676]
    See all SNRPD3/Sm-D3 primary antibodies
  • Description

    Rabbit monoclonal [EPR7676] to SNRPD3/Sm-D3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human SNRPD3/Sm-D3 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Jurkat, 293T and Caco-2 cell lysates; 293T cells NIH/3T3 and HEK-293 lysates.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as SNRPD3. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7676
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Images

  • Western blot - Anti-SNRPD3/Sm-D3 antibody [EPR7676] (ab128857)
    Western blot - Anti-SNRPD3/Sm-D3 antibody [EPR7676] (ab128857)
    All lanes : Anti-SNRPD3/Sm-D3 antibody [EPR7676] (ab128857) at 1/5000 dilution (purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14 kDa



    Blocking and diluting buffer: 5% NFDM/TBST. 

  • Western blot - Anti-SNRPD3/Sm-D3 antibody [EPR7676] (ab128857)
    Western blot - Anti-SNRPD3/Sm-D3 antibody [EPR7676] (ab128857)
    Anti-SNRPD3/Sm-D3 antibody [EPR7676] (ab128857) at 1/1000 dilution (purified) + NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 14 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-SNRPD3/Sm-D3 antibody [EPR7676] (ab128857)
    Western blot - Anti-SNRPD3/Sm-D3 antibody [EPR7676] (ab128857)
    All lanes : Anti-SNRPD3/Sm-D3 antibody [EPR7676] (ab128857) at 1/1000 dilution (unpurified)

    Lane 1 : Jurkat cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Caco-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 14 kDa

  • Anti-SNRPD3/Sm-D3 antibody [EPR7676] (ab128857)
    Anti-SNRPD3/Sm-D3 antibody [EPR7676] (ab128857)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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