Anti-Histone H3 (acetyl K9 + K14) antibody (ab272151)
Key features and details
- Rabbit polyclonal to Histone H3 (acetyl K9 + K14)
- Suitable for: ICC/IF, Dot blot, WB
- Reacts with: Mouse, Human, Caenorhabditis elegans
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (acetyl K9 + K14) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (acetyl K9 + K14) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Dot blot, WBmore details -
Species reactivity
Reacts with: Mouse, Human, Caenorhabditis elegans -
Immunogen
Synthetic peptide corresponding to Human Histone H3 (acetyl K9 + K14).
Database link: Q71DI3 -
Positive control
- WB: NIH/3T3 histone preps; C.elegans embryo lysate. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9 + K14) antibody (ab272151)
Immunofluorescence analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (acetyl K9 + K14) (green) with ab272151 at 5 µg/ml overnight at 2-8°C, followed by Goat Anti-Rabbit IgG DyLight™488 at 5 µg/ml for 1 hour at room temperature.
Nuclear Counterstain: DAPI. Actin Filament Stain: Texas Red - X Phallodin.
Staining: (A) DAPI. (B) ab272151 + DyLight™488. (C) Actin-X Phallodin. (D) Merge A+B. (E) Merge A+B+C. (F) Secondary Only Merge.
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Western blot - Anti-Histone H3 (acetyl K9 + K14) antibody (ab272151)Anti-Histone H3 (acetyl K9 + K14) antibody (ab272151) at 1/500 dilution + NIH/3T3 (mouse embryo fibroblast cell line) histone preps at 30 µg
Secondary
IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9 + K14) antibody (ab272151)Immunofluorescence analysis of 0.5% PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (acetyl K9 + K14) (green) with ab272151 at 1/200 dilution for 1 hour at room temperature, followed by FITC secondary antibody at 1/10000 for 45 minutes at room temperature. Nuclei and actin are counterstained with Dapi (blue) and Phalloidin (red).
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Western blot - Anti-Histone H3 (acetyl K9 + K14) antibody (ab272151)Anti-Histone H3 (acetyl K9 + K14) antibody (ab272151) at 1/500 dilution + C.elegans embryo lysate at 30 µg
Secondary
IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDa
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Dot Blot - Anti-Histone H3 (acetyl K9 + K14) antibody (ab272151)Dot Blot of ab272151.
Lane 1: K9-K14.
Lane 2: K9-KAc-K14-Kac.
Lane 3: K9-K14-Kac.
Lane 4: K9-Ac-K14.
Load: 0.3, 1, and 3 µg of peptide. Primary antibody ab272151 for 45 min at 4°C. HRP rabbit secondary antibody at 1/10000 for 45 minutes at room temperature.