Anti-Histone H3 (tri methyl K27, phospho S28) antibody (ab272155)
Key features and details
- Rabbit polyclonal to Histone H3 (tri methyl K27, phospho S28)
- Suitable for: WB, ChIP, Dot blot, ICC/IF
- Reacts with: Mouse, Human, Caenorhabditis elegans
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (tri methyl K27, phospho S28) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (tri methyl K27, phospho S28) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ChIP, Dot blot, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human, Caenorhabditis elegans -
Immunogen
Recombinant fragment corresponding to Human Histone H3 (tri methyl L27, phospho S28).
Database link: Q71DI3 -
Positive control
- ChIP: Chromatin prepared from HeLa cells. WB: HeLa histone preparation;, NIH/3T3 histone preparation; Caenorhabditis elegans embryo lysate. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H3 (tri methyl K27, phospho S28) antibody (ab272155)All lanes : Anti-Histone H3 (tri methyl K27, phospho S28) antibody (ab272155) at 1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) histone preparation
Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) histone preparation
Lane 3 : Caenorhabditis elegans embryo lysate
Predicted band size: 15 kDaPrimary incubation overnight at 4°C.
Block: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
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ChIP - Anti-Histone H3 (tri methyl K27, phospho S28) antibody (ab272155)ChIP analysis of chromatin from one million formaldehyde cross-linked HeLa (Human epithelial cell line from cervix adenocarcinoma) cells using 2µg of ab272155 and 20µl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27, phospho S28) antibody (ab272155)Immunofluorescent analysis of 0.5% PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (tri methyl K27, phospho S28) using ab272155 at a 1/2000 dilution for 1 hour at room temperature (Green). Dylight 488 secondary antibody at 1/10,000 for 45 minutes at room temperature. The nuclear counter stain is DAPI (Blue). Alpha tubulin is shown in red.
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Dot Blot - Anti-Histone H3 (tri methyl K27, phospho S28) antibody (ab272155)Dot Blot analysis of Histone H3 (tri methyl K27, phospho S28) using ab272155 at 1/1000 dilution.
Lane 1: pS28/K27 unmodified.
Lane 2: pS28 N-Term.
Lane 3: p28 C-term.
Lane 4: K27Me3.
Lane 5: pS28/K27Me3.
Load: 1, 10, and 100 picomoles of peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K27, phospho S28) antibody (ab272155)Immunofluorescent analysis of 0.5% PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (tri methyl K27, phospho S28) using ab272155 at a 1/2000 dilution for 1 hour at room temperature. Dylight 488 secondary antibody at 1/10,000 for 45 minutes at room temperature. The nuclear counter stain is DAPI (Blue). Alpha tubulin is shown in red.