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Human RHOT1 (MIRO1) knockout HeLa cell pellet (ab279201)

Overview

  • Product name

    Human RHOT1 (MIRO1) knockout HeLa cell pellet
    See all MIRO1 kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HeLa

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.

  • Passage number

  • Knockout validation

    Sanger Sequencing

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Properties

Target

  • Function

    Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.

  • Tissue specificity

    Ubiquitously expressed. Expressed at high level in heart and skeletal muscle.

  • Sequence similarities

    Belongs to the mitochondrial Rho GTPase family.
    Contains 2 EF-hand domains.
    Contains 2 Miro domains.

  • Cellular localization

    Mitochondrion outer membrane. Colocalizes with MGARP and RHOT2 at the mitochondria.
  • Information by UniProt

  • Alternative names

    • ARHT 1
    • ARHT1
    • FLJ11040
    • FLJ12633
    • hMiro 1
    • hMiro-1
    • hMiro1
    • MIRO 1
    • MIRO-1
    • MIRO1
    • MIRO1_HUMAN
    • mitochondrial Rho (MIRO) GTPase 1
    • Mitochondrial Rho 1
    • Mitochondrial Rho GTPase 1
    • Mitochondrial Rho1
    • Rac GTP binding protein like protein
    • Rac-GTP-binding protein-like protein
    • Ras homolog family member T1
    • Ras homolog gene family member T1
    • Rhot 1
    • RHOT1
    see all

Properties

Images

  • Sanger Sequencing - Human RHOT1 (MIRO1) knockout HeLa cell pellet (ab279201)
    Sanger Sequencing - Human RHOT1 (MIRO1) knockout HeLa cell pellet (ab279201)
    Homozygous: 1 bp insertion in exon1

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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