Alkaline Phosphatase Anti-6X His tag® antibody [HIS-1] (ab49746)
Key features and details
- Alkaline Phosphatase Mouse monoclonal [HIS-1] to 6X His tag®
- Suitable for: WB, Dot blot, ELISA
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG2a
Overview
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Product name
Alkaline Phosphatase Anti-6X His tag® antibody [HIS-1]
See all 6X His tag® primary antibodies -
Description
Alkaline Phosphatase Mouse monoclonal [HIS-1] to 6X His tag® -
Host species
Mouse -
Conjugation
Alkaline Phosphatase -
Tested applications
Suitable for: WB, Dot blot, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant poly(Histidine)-tagged fusion protein.
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Positive control
- Bacteria lysates expressing a recombinant polyHistidine tagged fusion protein and BCIP/NBT substrate.
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General notes
HIS-TAG® is a trademark of EMD Biosciences, Inc.
Monoclonal antibodies reacting specifically with poly-histidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidinetagged fusion protein expressing vectors.
The antibody is prepared by conjugation of calf intestinal alkaline phosphatase to mouse monoclonal anti-polyHistidine purified from ascites fluid of the HIS-1 hybridoma
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.00
Preservative: 0.097% Sodium azide
Constituents: 0.06% Tris buffered saline, 0.0095% Magnesium chloride, 50% Glycerol, 1% BSA -
Concentration information loading... -
Primary antibody notes
Monoclonal antibodies reacting specifically with poly-histidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidinetagged fusion protein expressing vectors. -
Clonality
Monoclonal -
Clone number
HIS-1 -
Isotype
IgG2a -
Research areas
