Human FOLR3 ELISA Kit (ab267613)
Key features and details
- Sensitivity: 32 pg/ml
- Range: 32.77 pg/ml - 8000 pg/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human FOLR3 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
32 pg/ml -
Range
32.77 pg/ml - 8000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 103 96% - 110% Plasma 96 86% - 108% Cell culture media 91 89% - 95% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human FOLR3 ELISA Kit is designed for the quantitative determination of FOLR3 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human FOLR3 coated on a 96-well plate. Standards and samples are pipetted into the wells and FOLR3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human FOLR3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FOLR3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Human FOLR3 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human FOLR3 Detection Antibody 2 vials Human FOLR3 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Function
Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. Isoform Short does not bind folate. -
Tissue specificity
Spleen, thymus, bone marrow, ovarian carcinoma, and uterine carcinoma. -
Sequence similarities
Belongs to the folate receptor family. -
Post-translational
modificationsEight disulfide bonds are present. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Folate receptor 3
- Folate receptor 3 (gamma)
- Folate receptor gamma
see all -
Database links
- Entrez Gene: 2352 Human
- Omim: 602469 Human
- SwissProt: P41439 Human
- Unigene: 352 Human
Images
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Standard curve
Human FOLR3 ELISA kit (ab267613) Standard curve.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.