Anti-NF-kB p65 (phospho S529) antibody (ab97726)
Key features and details
- Rabbit polyclonal to NF-kB p65 (phospho S529)
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NF-kB p65 (phospho S529) antibody
See all NF-kB p65 primary antibodies -
Description
Rabbit polyclonal to NF-kB p65 (phospho S529) -
Host species
Rabbit -
Specificity
ab97726 detects endogenous levels of NFkB p65 protein only when phosphorylated at serine 529. -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human NF-kB p65.
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Positive control
- Human breast carcinoma tissue; 293 cell extracts, treated with TNF alpha; HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab97726 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatogramphy using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NF-kB p65 (phospho S529) antibody (ab97726)All lanes : Anti-NF-kB p65 (phospho S529) antibody (ab97726) at 1/500 dilution
Lane 1 : 293 cell extracts, untreated
Lane 2 : 293 cell extracts, treated with TNF alpha
Predicted band size: 60 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 (phospho S529) antibody (ab97726)ab97726 at 1/100 dilution staining methanol-fixed HeLa cells by Immunofluorescence (seen in red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 (phospho S529) antibody (ab97726)ab97726 at 1/50 dilution staining NF-kB p65 in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue in the absence or presence of the immunising peptide.
