Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RanBP3 antibody [EPR5088(2)] (ab134052)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5088(2)] to RanBP3
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-RanBP3 antibody [EPR5088(2)]
    See all RanBP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR5088(2)] to RanBP3

  • Host species

    Rabbit

  • Specificity

    The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human RanBP3 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: HEK-293, MEF, and Y79 cell lysates; IHC: Human clear cell carcinoma of kidney, Human testis tissue.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP3 antibody [EPR5088(2)] (ab134052)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP3 antibody [EPR5088(2)] (ab134052)

    Immunohistochemistry of Human clear cell carcinoma of kidney tissues staining RanBP3 using ab134052 (1/1000). a HRP conjugated Goat Anti-Rabbit IgG H&L was used as a secondary antibody. 

    Antigen retrieval performed using heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    image shows nuclear and cytoplasmic staining on human clear cell carcinoma of kidney.

  • Western blot - Anti-RanBP3 antibody [EPR5088(2)] (ab134052)
    Western blot - Anti-RanBP3 antibody [EPR5088(2)] (ab134052)
    All lanes : Anti-RanBP3 antibody [EPR5088(2)] (ab134052) at 1/1000 dilution

    Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 2 : MEF (Mouse embryonic fibroblast) whole cell lysates
    Lane 3 : Y79 (Human retinoblastoma retinoblastoma) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/200000 dilution

    Predicted band size: 60 kDa
    Observed band size: 70,90 kDa
    why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    The double bands are consistent with what has been described in PMID 16314428.

    We are unsure how to define the extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP3 antibody [EPR5088(2)] (ab134052)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP3 antibody [EPR5088(2)] (ab134052)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling RanBP3 with ab134052 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-RanBP3 antibody [EPR5088(2)] (ab134052)
    Anti-RanBP3 antibody [EPR5088(2)] (ab134052)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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