Anti-RanBP3 antibody (ab241502)
Key features and details
- Rabbit polyclonal to RanBP3
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RanBP3 antibody
See all RanBP3 primary antibodies -
Description
Rabbit polyclonal to RanBP3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide within Human RanBP3 aa 150-200. The exact sequence is proprietary. NP_015561.1
Database link: Q9H6Z4 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysate. IP: RanBP3 IP in HeLa whole cell lysate. IHC-P: Human ovarian carcinoma and colon carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to RanBP3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RanBP3 antibody (ab241502)All lanes : Anti-RanBP3 antibody (ab241502) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-239T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Predicted band size: 60 kDa
Exposure time: 10 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP3 antibody (ab241502)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for RanBP3 with ab241502 at 1/200 dilution in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP3 antibody (ab241502)Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for RanBP3 with ab241502 at 1/80 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-RanBP3 antibody (ab241502)RanBP3 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab241502 used for IP at 3 µg/mg lysate. For WB 1 µg/ml.
Lane 1: ab241502 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Chemiluminescence detection: 3 seconds.
